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KMID : 0624219940020000043
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Focus on Genetic Science
1994 Volume.2 No. 0 p.43 ~ p.53
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Expression of Human Blood Coagulation Factor Ⅷ in Chinese Hamster Ovary Cells
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Oh Sang-Hwan
Kim Seok-Hyun
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Abstract
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The synthesis and secretion of a biologically active human blood coagulation factor Ⅷ (FⅧ) in eukaryotic cell culture system has been designed. B-domain deleted FⅧ cDNA inserted into pCMVβ plasmid was cotransfected with pRSVneo into Chinese hamster ovary (CHO) cells for the production of FⅧ protein. The expression of B-domain deleted FⅧ mRNA was detected by reverse transcriptase polymerase chain reaction (RT-RCR), and the presence of a biologically active FⅧ protein in the culture media of cells was confirmed. These results indicate that the expression of FⅧ cDNA in eukaryotic cells may be applicable for the production of biologically active protein.
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